๐ช๐ต๐ ๐ณ๐ฌ%๐๐ฃ๐[๐ถ๐๐ผ๐ฝ๐ฟ๐ผ๐ฝ๐๐น ๐ฎ๐น๐ฐ๐ผ๐ต๐ผ๐น] ๐๐๐ฒ ๐ถ๐ป ๐ฝ๐ต๐ฎ๐ฟ๐บ๐ฎ๐ฐ๐ฒ๐๐๐ถ๐ฐ๐ฎ๐น ๐ถ๐ป๐ฑ๐๐๐๐ฟ๐ถ๐ฒ๐ ๐ฎ๐ ๐ฑ๐ถ๐๐ถ๐ป๐ณ๐ฒ๐ฐ๐๐ฎ๐ป๐ ๐ถ๐ป๐๐๐ฒ๐ฎ๐ฑ ๐ผ๐ณ ๐ญ๐ฌ๐ฌ% ๐๐ฃ๐ ๐๐๐ฒ๐ฑ. ๐๐ต๐ ๐ณ๐ฌ% ๐๐ฃ๐ ๐ถ๐ ๐บ๐ผ๐ฟ๐ฒ ๐ฒ๐ณ๐ณ๐ฒ๐ฐ๐๐ถ๐๐ฒ ๐ฑ๐ถ๐๐ถ๐ป๐ณ๐ฒ๐ฐ๐๐ฎ๐ป๐? ๐๐๐ฒ ๐ผ๐ณ ๐ณ๐ฌ% ๐๐ฝ๐ฎ
What is 70%Isopropyl alcohol?
70ml ipa and 30 ml purified water and make volume 100 ml that is 70% isopropyl alcohol.
[A]. WHy 70% Isopropyl alcohol is use in pharmaceutical industries as a disinfectant? Whya 91% is not used? 70%Ipa is common and most widely using disinfectant in the pharmaceutical industries, hospitals and other health care facilities.
70%ipa is used for disinfection of hands and equipment surface and surgical devices.
70%Ipa is only acts as a disinfectant killing all surface microorganisms.
70 %Ipa is kills microorganisms by dissolving the plasma membrane of the cell wall. Plasma membrane of gram negative bacteria consist of thin layer of peptidoglycon that easily destroyed by the ipa.
Water plays key important role which used to denature the proteins of cell membrane and acts as a catalyst in the reaction. Contact time of the Ipa with the organism also play an important role.
70% ipa takes more time in evaporation from the surface, increasing the contact time. Therefore, 70% ipa fulfills the both requirements.
The 91% isopropyl alcohol coagulates the proteins instantly by creating a protein layer that protects the other proteins from further coagulation.
Due to this microbes are not killed but remains in dormant stage.
While 70%Ipa solution penetrates in the cell wall at slower rate and coagulates the all protein of the cell wall and microorganism dies.
For eg.Staphylococcus aureus killed in less than 10 seconds with a 50% Isopropyl Alcohol solution and was not killed in 2 hours with a 90% solution: emphasizing the importance of water in killing microorganisms.
Thus 70% IPA solution in water is more effective then 91% Ipa and have more disinfectant capacity. Hence 70% IPA is known as pharmaceutical alcohol.
[B].Why 70% ipa is more effective disinfectant instead of other concentration.?
Isopropyl alcohol are rapidly bactericidal rather than bacteriostatic against vegetative forms of bacteria they also are tuberculocidal, fungicidal, and virucidal but do not destroy bacterial spores.
Addition of water [due to high dielectric constant] to alcohol breaks the hydrogen bonds [due to hydrogen bond, alcohol molecules are inactive] between the alcohol molecules and make molecules active. There is a critical concentration of water molecules which makes maximum free alcohol molecules. 30% water concentration is critical concentration for isopropyl alcohol therefore, 70%isopropyl alcohol is more effective and use as a disinfectant.
For eg. Staphylococcus aureus killed in 1 minute; Escherichia coli killed in 5 minutes ; spored cultures of Bacillus subtilis and Clostridium novyi not killed in 60 minutes; alcohol contaminated by saprophytic spore-forming organism does not kill bacterial spores.
Isopropyl alcohol have been excluded as high-level disinfectants because of their inability to inactivate bacterial spores and because of the inability of isopropyl alcohol to inactivate hydrophilic viruses [I.e poliovirus, coxsackie ]
[C]. Why 70% ipa is use for sanitization?
70% isopropyl alcohol is most commonly used disinfectant in pharmaceutical industries.
The important thing is that only 70% of isopropyl alcohol acts as a disinfectant killing all surface microorganisms.
70 % isopropyl alcohol solution kills microorganisms by dissolving plasma membrane of the cell wall.
Hence 70%ipa is use for sanitization.
[D]. Isopropyl alcohol advantage and disadvantage on skin.
Isopropyl alcohol is readily absorbed through the skin, so spilling large amounts of IPA on the skin may cause accidental poisoning. Small amounts of IPA on the skin is generally not dangerous, but repeated skin exposure can cause itching, redness, rash, drying, and cracking. Prolonged skin contact may cause corrosion.
[E]. What is an expiration date of 70% isopropyl alcohol.
Rubbing alcohol has an expiration date Rubbing alcohol expires because isopropanol evaporates when exposed to the air, while the water remains. As a result, the percentage of isopropanol can decrease over time, making it less effective.
[F] While using 70% ipa following points should bhe cindired.
1. 70% IPA should be prepared on daily basis.
2.70% IPA preparation should be done in controlled area.
3.when the preparation of the 70%ipa , purified water should be used.
4.70%ipa use shold be freshly prepared and proper labeled like name of solution , date of preparation and sign of prepared by.
5.The bulk container of 70% ipa sholud have proper lable with the details like name of solution, approval date and checked by date and sign it is used for distribution.
[G].Use of 70%Isopropyl alcohol
1. 70%ipa is use for disinfectant purpose in pharmaceutical companies.
2. 70%ipa is use for the sanitize the hands.
3. 70%ipa is use for equipment surface cleaning pupose.
4.70%ipa quickly works against harmful bacteria, fungi, and viruses, relibly destroying cells on a wide range of different surfaces.
5. 70%ipa is very effective sanitizer for kiliing covide -19 bacteria.
[H]. Use of 91 % Isopropyl alcohol
1. 91% ipa is use to clean cuts.
2.91% ipa use to burns on the skin and to promote healing.
3.91%ipa is use in medical scenarious.
4.91%ipa is ideal use for those cuts and scrapes that require a quick clean.
5.91%ipa is commonly use to sterilize skin and needles prior to injections.
6.91%ipa is perfect for those who must administer injections regularly like those with diabetes for example.
7.91%ipa is use on top of stubborn stains and marks around the house or in the work place.
8.91%ipa is a powerful liquid which is highly effective against marks and stains, working quickly to remove marks minimal effort.
Author BY: Nilam Gawankar ( Microbiologist)
Nilmaypharmablogg
Published on 17/09/2022
Reference by:guidelines and science base reaserch
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